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flowsight® imaging flow cytometer  (Merck KGaA)

 
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    Merck KGaA flowsight® imaging flow cytometer
    Flowsight® Imaging Flow Cytometer, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flowsight%C2%AE+imaging+flow+cytometer/pmc12085582-156-11-17?v=Merck+KGaA
    Average 90 stars, based on 1 article reviews
    flowsight® imaging flow cytometer - by Bioz Stars, 2026-07
    90/100 stars

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    CSCs sorting process. A . Flow cytometry was used to detect CD133 + , CD166 + , and CD133 + /CD166 + CSCs populations within mixed cells, with the R2 gate serving as the target cell population. B . Sorting of CSCs from three tissue classes using the cartridge of the MACSQuant Tyto system, with the cartridge and microchip on the left and cell sorting through the microchip on the right. C . Real-time visualization of CSCs obtained by <t>FlowSight</t> imaging flow cytometer. Bright field and cell fluorescence image are presented, with Ch01: light field, Ch03: CD133-PE, and Ch11: CD166-BV421; scale bar = 20 μm. Flow cytometric analysis was performed on the sorted negative cells
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    Lungs were harvested from binge-on-chronic alcohol-fed mice with and without treatment post K. pneumoniae infection and the percentage and phenotype of pulmonary NK cells was assessed. The percentage of ( A ) NK1.1 cells, ( B ) AhR+ NK cells, ( C ) TGF-βR1 + NK cells, and ( D ) percentage of pulmonary NK cells with nuclear localization of AhR, was assessed using Anims <t>FlowSight.</t> p values are indicated by one‐way ANOVA with Sidak’s multiple comparisons. N = 6–14 mice/group derived from 2 independent experiments.
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    Lungs were harvested from binge-on-chronic alcohol-fed mice with and without treatment post K. pneumoniae infection and the percentage and phenotype of pulmonary NK cells was assessed. The percentage of ( A ) NK1.1 cells, ( B ) AhR+ NK cells, ( C ) TGF-βR1 + NK cells, and ( D ) percentage of pulmonary NK cells with nuclear localization of AhR, was assessed using Anims <t>FlowSight.</t> p values are indicated by one‐way ANOVA with Sidak’s multiple comparisons. N = 6–14 mice/group derived from 2 independent experiments.
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    Image Search Results


    CSCs sorting process. A . Flow cytometry was used to detect CD133 + , CD166 + , and CD133 + /CD166 + CSCs populations within mixed cells, with the R2 gate serving as the target cell population. B . Sorting of CSCs from three tissue classes using the cartridge of the MACSQuant Tyto system, with the cartridge and microchip on the left and cell sorting through the microchip on the right. C . Real-time visualization of CSCs obtained by FlowSight imaging flow cytometer. Bright field and cell fluorescence image are presented, with Ch01: light field, Ch03: CD133-PE, and Ch11: CD166-BV421; scale bar = 20 μm. Flow cytometric analysis was performed on the sorted negative cells

    Journal: Journal of Translational Medicine

    Article Title: Comparison of functional characterization of cancer stem cells in different tumor tissues of pseudomyxoma peritonei

    doi: 10.1186/s12967-024-05730-6

    Figure Lengend Snippet: CSCs sorting process. A . Flow cytometry was used to detect CD133 + , CD166 + , and CD133 + /CD166 + CSCs populations within mixed cells, with the R2 gate serving as the target cell population. B . Sorting of CSCs from three tissue classes using the cartridge of the MACSQuant Tyto system, with the cartridge and microchip on the left and cell sorting through the microchip on the right. C . Real-time visualization of CSCs obtained by FlowSight imaging flow cytometer. Bright field and cell fluorescence image are presented, with Ch01: light field, Ch03: CD133-PE, and Ch11: CD166-BV421; scale bar = 20 μm. Flow cytometric analysis was performed on the sorted negative cells

    Article Snippet: Cells were acquired using a FlowSight imaging flow cytometer (Amnis, part of EMD Millipore, Massachusetts, USA), and cell debris and dead cells were identified and removed based on the aspect ratio and area of the cells.

    Techniques: Flow Cytometry, MicroChIP Assay, FACS, Imaging, Fluorescence

    Lungs were harvested from binge-on-chronic alcohol-fed mice with and without treatment post K. pneumoniae infection and the percentage and phenotype of pulmonary NK cells was assessed. The percentage of ( A ) NK1.1 cells, ( B ) AhR+ NK cells, ( C ) TGF-βR1 + NK cells, and ( D ) percentage of pulmonary NK cells with nuclear localization of AhR, was assessed using Anims FlowSight. p values are indicated by one‐way ANOVA with Sidak’s multiple comparisons. N = 6–14 mice/group derived from 2 independent experiments.

    Journal: NPJ Biofilms and Microbiomes

    Article Title: Natural killer cell effector function is critical for host defense against alcohol-associated bacterial pneumonia

    doi: 10.1038/s41522-024-00558-w

    Figure Lengend Snippet: Lungs were harvested from binge-on-chronic alcohol-fed mice with and without treatment post K. pneumoniae infection and the percentage and phenotype of pulmonary NK cells was assessed. The percentage of ( A ) NK1.1 cells, ( B ) AhR+ NK cells, ( C ) TGF-βR1 + NK cells, and ( D ) percentage of pulmonary NK cells with nuclear localization of AhR, was assessed using Anims FlowSight. p values are indicated by one‐way ANOVA with Sidak’s multiple comparisons. N = 6–14 mice/group derived from 2 independent experiments.

    Article Snippet: For all experiments, cells were acquired using an Amnis® FlowSight® Imaging Flow Cytometer (Millipore Sigma, Burlington, MA).

    Techniques: Infection, Derivative Assay